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Cidar_L1

Output Separator : Dot ; Restriction Enzyme : BsaI

Promoter

RBS

Gene

Terminator

Backbone

Cidar_L2

Output Separator : Tilde ; Restriction Enzyme : BbsI

TU1

TU2

TU3

TU4

Backbone

Ecoflex_L1

Output Separator : Hyphen ; Restriction Enzyme : BsaI

Promoter

CDS

Terminator

Backbone

Ecoflex_L2

Output Separator : Underscore ; Restriction Enzyme : BsmBI

TU1a

TU1b

TU1c

TU1d

TU1e

Backbone

Ecoflex_L3

Output Separator : Underscore ; Restriction Enzyme : BsaI

TU2a

TU2b

TU2c

TU2d

Backbone

Expyali_L1

Output Separator : Hyphen ; Restriction Enzyme : BsaI

Promoter

Gene

Terminator

Backbone

Expyali_L2

Output Separator : Underscore ; Restriction Enzyme : BsmBI

Cassette Plasmid A

Cassette Plasmid B

Cassette Plasmid C

Backbone Multi

Ftk

Output Separator : Hyphen ; Restriction Enzyme : BsaI

Promoter

CDS

Terminator

Backbone

Moclo_Ppk_L1

Output Separator : Hyphen ; Restriction Enzyme : BsaI

Pro5U

CDS

CT

3UTer

Backbone

Moclo_Ppk_L2

Output Separator : Underscore ; Restriction Enzyme : BbsI

TU1

TU2

TU3

TU4

TU5

TU6

TU7

End linker

Backbone

Moclo_Ppk_Lm

Output Separator : Underscore ; Restriction Enzyme : BbsI

TU1

TU2

TU3

TU4

TU5

TU6

TU7

End linker

Backbone

Openpichia_L1

Output Separator : Hyphen ; Restriction Enzyme : BsaI

ConUp

Promoter

Gene

Terminator

ConDown

Marker

ARS-integration

Backbone

Raw_Bsai

Output Separator : Hyphen ; Restriction Enzyme : BsaI

input Plasmid 1

input Plasmid 2

input Plasmid 3

input Plasmid 4

input Plasmid 5

input Plasmid 6

input Plasmid 7

input Plasmid 8

Yali_Gg

Output Separator : Hyphen ; Restriction Enzyme : BsaI

InsertUp

Marker

Prom1

Gene1

Term1

Prom2

Gene2

Term2

Prom3

Gene3

Term3

InsertDown

Backbone

Ytk_L1

Output Separator : Hyphen ; Restriction Enzyme : BsaI

ConL

Promoter

Gene

Terminator

ConR

Backbone

Ytk_L2

Output Separator : Underscore ; Restriction Enzyme : BsmBI

TU1

TU2

TU3

TU4

TU5

TU6

Backbone